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our science

ENHANCE PROTEIN

BIOGENESIS

We enhance protein expression in CHO cells by optimizing secretion at the ER level – moving signal peptide optimization out of manual, trial-and-error testing into high-throughput engineering.

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Mechanism

IT STARTS AT THE N TERMINUS

Essentially every therapeutic protein manufactured today begins with a signal peptide: a short sequence at its N terminus that directs the nascent chain into the endoplasmic reticulum, where folding and assembly begin.

Signal peptide

Cleavage site

Mature protein

MAJOR IMPACT ON EXPRESSION

The signal peptide can strongly influence how efficiently a protein enters the secretory pathway, and ultimately, how much is produced and secreted.

BEYOND AN ADDRESS TAG

Signal peptides can influence downstream processes including folding, disulfide bond formation, glycosylation and trafficking.

CLEAVED OFF

Typically removed during protein biogenesis, supporting biosimilarity.

PRODUCT-SPECIFIC

The best-performing signal peptide depends on the target protein. A sequence that works well for one may perform poorly for another.

VAST SEQUENCE SPACE

Nature provides enormous signal peptide diversity; synthetic design expands the possibilities further.

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Platform

A powerful combination of machine learning and wet lab chemistry

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Library

Large SP libraries tailored specifically for your target of interest.

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Display

Our technology measures protein secretion efficiency at individual cell level.

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DNA Analysis

Identifying SP-protein combinations that drive the highest secretory output.

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Validation

Top candidates characterized for enhanced expression and quality.

APPLICATION POSSIBILITIES

ANTIBODIES

FUSION PROTEINS

CELL & GENE THERAPIES

mRNA THERAPIES

ENZYMES

AI-DESIGNED PROTEINS

Technology

AN UNPARALLELED COMBINATION OF YIELD AND QUALITY

N-terminal signal peptide engineering is an essential part of our protein engineering platform. We offer a robust, high throughput strategy to tailor the most optimal molecular sequence to drive significant increase in protein production efficacy without compromising the quality of the product.

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This technology enables our customers, for the first time, to maximize the value of protein biogenesis on endoplasmic reticulum level, leading to enhancements on protein folding, post-translational modifications and significantly enhanced expression.

External validation

BACKED UP BY RIGOROUS VALIDATION

Our platform has undergone rigorous external validation with numerous commercially valuable proteins. In all tested cases, the result has been significant boosting of expression yield when comparing to previously used strategies. Our results have also been independently validated in external biotechnology laboratories. The yield improvement has been replicated in stable and transient cell cultures, in different cell culture volumes, in different cell strains and by different laboratories – from milliliter to liter volume in standard industrial setting.

FROM ROADBLOCKS TO BREAKTHROUGHS

Problem

Protein expression is still a major bottleneck in the biotechnology industry. Despite all the advancements, for most proteins, production optimizations remain a long and expensive process especially in difficult-to express (DTE) proteins.

Solution

Our platform offers a unique solution to harness the full power of endoplasmic reticulum level regulation of protein biogenesis. We tailor unique molecular sequences that drive improved outcomes in protein production, from DTEs to any protein production optimization.

Machine learning and faster discovery in biotechnology.

REVOLUTIONIZING HEALTHCARE THROUGH INNOVATIVE SOLUTIONS

Discover our cutting-edge technology and services that drive advancements in biopharma.