SINGLE-CHAIN
Single-chain SP screening across large libraries of variants — identifying the best-performing signal peptide for any therapeutic protein.
TWO-CHAIN
Two different chains optimized in combination for balanced assembly. Built for formats such as antibodies.
CUSTOM
Complex targets, such as 3-chain or membrane, warrant a bespoke screen built around them.
Align — We define your target protein and objectives, receive the sequence, and design the screening strategy.
Build — We construct a customized library of thousands of signal peptide–protein variants for your target. This phase includes gene synthesis and cloning – required laboratory steps with their own turnaround.
Screen — The library is expressed in CHO cells and run through our assay, isolating the highest-producing cell clones, then deconvoluted by NGS analysis to identify hyper-performing combinations.
Validate & report — Lead candidates undergo validation — typically secretion titers, intact-mass and cleavage confirmation by mass spectrometry, plus assembly and quality analysis by SEC-MALS — delivered with a report and recommended signal peptides for your target.
Simultaneously analyzes thousands of naturally occurring and synthetic signal peptides
Delivers significant improvements in protein yield
Especially powerful for difficult-to-express proteins that hinder development
Compatible with expression optimization of any mammalian protein production system: from recombinant protein therapies to cell and gene therapies
